Journal: Life

Article Title: Potential Use of CTCs as Biomarkers in Renal Cancer Patients

doi: 10.3390/life12010089

Figure Lengend Snippet: A schematic presentation of the CellCollector. Straight ( a ) and spiraled ( b ) medical wire covered with anti-EpCAM (blue) and/or anti-MUC1 (black) antibody.

Article Snippet: Incubation with the anti-MUC1 mouse antibody (Cell Signaling) was performed overnight at 4 °C.

Techniques:

Journal: Life

Article Title: Potential Use of CTCs as Biomarkers in Renal Cancer Patients

doi: 10.3390/life12010089

Figure Lengend Snippet: The expression analysis of the renal normal and tumor tissues. ( a ) The transcriptome microarray analysis with a detailed description of the differentially expressed surface proteins; FPKM—Fragments Per Kilobase Million; ( b ) the protein expression of EpCAM, MUC1 and cytokeratin (green) in the clear cell renal tumor (pT3b) and corresponding control tissues. The nuclei were stained with Hoechst (blue).

Article Snippet: Incubation with the anti-MUC1 mouse antibody (Cell Signaling) was performed overnight at 4 °C.

Techniques: Expressing, Microarray, Control, Staining

Journal: Life

Article Title: Potential Use of CTCs as Biomarkers in Renal Cancer Patients

doi: 10.3390/life12010089

Figure Lengend Snippet: The surface markers in the primary ccRCC cell lines. ( a ) The transcriptome microarray analysis with a detailed description of EpCAM and MUC1 in ccRCC31, ccRCC45, ccRCC122 and ccRCC162; FPKM—Fragments Per Kilobase Million, ( b ) the localization of EpCAM (green) and MUC1 (red) proteins on the surface of the ccRCC45 separate cells and merged cells to visualize the colocalization. The images show protein localization. The nuclei were stained with Hoechst (blue).

Article Snippet: Incubation with the anti-MUC1 mouse antibody (Cell Signaling) was performed overnight at 4 °C.

Techniques: Microarray, Staining

Journal: Life

Article Title: Potential Use of CTCs as Biomarkers in Renal Cancer Patients

doi: 10.3390/life12010089

Figure Lengend Snippet: The statistically significant differential expression of EpCAM and MUC1 was determined with a microarray. All analyzed cell lines were compared with RCC45.

Article Snippet: Incubation with the anti-MUC1 mouse antibody (Cell Signaling) was performed overnight at 4 °C.

Techniques: Quantitative Proteomics, Microarray

Journal: Life

Article Title: Potential Use of CTCs as Biomarkers in Renal Cancer Patients

doi: 10.3390/life12010089

Figure Lengend Snippet: The CTCs captured by the spiraled wire. Both anti-EpCAM and anti-EpCAM/MUC1 antibody-covered CellCollector system wires were applied to the blood samples: G3 chromophiles (chRCC) and clear cells (pT3apNxpM1G2) (ccRCC). The attached cells were stained with antibodies against pan cytokeratin CK8, CK18 and CK19 (green) and CD45 (red). The nuclei were stained with Hoechst (blue).

Article Snippet: Incubation with the anti-MUC1 mouse antibody (Cell Signaling) was performed overnight at 4 °C.

Techniques: Staining

Journal: Life

Article Title: Potential Use of CTCs as Biomarkers in Renal Cancer Patients

doi: 10.3390/life12010089

Figure Lengend Snippet: The comparison of the CellCollector wires functionalized with either anti-EpCAM or anti-EpCAM/anti-MUC1 antibodies. ( a ) The number of CTCs captured by anti-EpCAM and anti-EpCAM/anti-MUC1 antibody-covered wires in the matched paired patient samples. Green line—lung metastasis, blue line—other metastases. ( b ) The presentation of CTC detection by both wires with a median of 4 (range 0–15) for anti-EpCAM-coated wires and a median of 6 (range 1–18) for anti-EpCAM/MUC1-coated wires. The mean is displayed as “+” and the whiskers represent the min and max values.

Article Snippet: Incubation with the anti-MUC1 mouse antibody (Cell Signaling) was performed overnight at 4 °C.

Techniques: Comparison